IB Biology HL A2.1 Origins of cells Questions

Practise IB Biology HL A2.1 with evidence-led questions on early-Earth chemistry, protocells, RNA as an early genetic material and radiometric dating.

20 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic9 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts11
Paper types5

Most tested objectives

  1. A2.1.6—RNA as presumed first genetic material3 papers
  2. A2.1.8—Dating first cells and LUCA3 papers
  3. A2.1.4—Evidence for origin of carbon compounds2 papers
  4. A2.1.5—Spontaneous formation of vesicles2 papers
  5. A2.1.1—Conditions on early Earth1 paper

Common question formats

  • Structured response
  • Process explanation
  • Definition or recall
  • Evaluation
  • Extended response

Recent exam appearances

November 2025Paper 2 HL · TZ3Q10(a)[ 5 ]
May 2025Paper 1A HL · TZ1Q12[ 1 ]
May 2022Paper 1 HL · TZ1Q3[ 1 ]

117 papers analysed · Tagging incomplete

Exam points

  • Evaluate early-Earth conditions and experimental evidence for abiotic synthesis of organic compounds, including suitable environments and the Miller–Urey findings.
  • Describe phospholipid-enclosed protobionts or vesicles as structures preceding cells and explain how a membrane creates a distinct internal chemical environment.
  • Explain how RNA structure, self-replication, catalytic activity and information storage support the RNA-first hypothesis.
  • Use carbon-14 and potassium-40 decay, isotope ratios and half-lives to date fossils or rocks and choose an isotope for the sample age.

Exam points

  • Describe the early Earth atmosphere, including volcanic gases, high carbon dioxide, methane, water vapour and little free oxygen, and explain the intense surface UV caused by a lack of ozone.
  • Outline how prebiotic chemistry may have formed amino acids, nitrogenous bases, sugars, fatty acids and nucleotides.

Question 1

[Maximum number: 3]

The synthesis of complex organic molecules in sea water is believed to be an important step in the evolution of life on Earth. Researchers investigated whether the evaporation of sea water containing amino acids could catalyse the formation of dipeptides such as divaline (valine-valine) under prebiotic Earth conditions. They placed different amino acid combinations in a chamber to simulate the evaporation cycles between high tides in shallow seas. In one investigation the amino acid valine was used as the substrate and the percentage yield of divaline was measured after different numbers of evaporation cycles. The experiment was repeated without a catalyst and with either glycine or histidine as catalysts.

Figure for Question 1 — IB Biology HL

This investigation was performed in a chamber at 85∘C85^{\circ} \mathrm{C} leading to total evaporation of the salt water within one day. The atmosphere inside the chamber consisted of nitrogen, carbon dioxide and water vapour. Evaluate the experiment on the basis of similarity with conditions that existed on the prebiotic Earth.

Exam points

  • Describe the Miller–Urey production of amino acids using methane, ammonia, hydrogen and water vapour.
  • Explain how later experiments using different gases and energy sources, including UV and ionizing radiation, support abiotic synthesis of amino acids, fatty acids, sugars, bases and simple polymers.

Question 1

[Maximum number: 2]

The synthesis of complex organic molecules in sea water is believed to be an important step in the evolution of life on Earth. Researchers investigated whether the evaporation of sea water containing amino acids could catalyse the formation of dipeptides such as divaline (valine-valine) under prebiotic Earth conditions. They placed different amino acid combinations in a chamber to simulate the evaporation cycles between high tides in shallow seas. In one investigation the amino acid valine was used as the substrate and the percentage yield of divaline was measured after different numbers of evaporation cycles. The experiment was repeated without a catalyst and with either glycine or histidine as catalysts.

Figure for Question 1 — IB Biology HL

Outline other possible locations where conditions could have allowed the synthesis of organic molecules under prebiotic conditions.

Exam points

  • Describe how amphipathic fatty acids can spontaneously form monolayers, bilayers and vesicles.
  • Explain how microspheres could enclose polymers and monomers to create internal chemistry, and that fatty acids likely preceded more complex phospholipid membranes.

Question 1

[Maximum number: 1]

Which cell component arose first during the formation of the earliest cells?

A

Chloroplast

B

Plasma membrane

C

Mitochondria

D

Cell wall

Exam points

  • Explain how RNA can store genetic information and fold into catalytic ribozymes, as proposed by the RNA world hypothesis before DNA and protein enzymes.
  • Discuss evidence for the RNA world hypothesis from ribosomal RNA catalysis, RNA primers and ribose formation under prebiotic conditions.

Question 1

[Maximum number: 5]

There is evidence that RNA was used as a genetic material in living organisms before DNA.

Describe the structure of RNA and the evidence that it was the first genetic material used to store information.

No public sample questions are available for this syllabus point yet.

Exam points

  • Describe how radiometric methods can date fossils and the rocks surrounding them.
  • Explain how DNA or protein changes in molecular clocks estimate divergence times and that LUCA may have existed around 4 billion years ago.

Question 1

[Maximum number: 2]

Outline the use of two named radioisotopes for dating fossils.

Question 1

[Maximum number: 5]

The synthesis of complex organic molecules in sea water is believed to be an important step in the evolution of life on Earth. Researchers investigated whether the evaporation of sea water containing amino acids could catalyse the formation of dipeptides such as divaline (valine-valine) under prebiotic Earth conditions. They placed different amino acid combinations in a chamber to simulate the evaporation cycles between high tides in shallow seas. In one investigation the amino acid valine was used as the substrate and the percentage yield of divaline was measured after different numbers of evaporation cycles. The experiment was repeated without a catalyst and with either glycine or histidine as catalysts.

Figure for Question 1 — IB Biology HL

Question (a)

(a)

This investigation was performed in a chamber at 85∘C85^{\circ} \mathrm{C} leading to total evaporation of the salt water within one day. The atmosphere inside the chamber consisted of nitrogen, carbon dioxide and water vapour. Evaluate the experiment on the basis of similarity with conditions that existed on the prebiotic Earth.

[ 3 ]

Question (b)

(b)

Outline other possible locations where conditions could have allowed the synthesis of organic molecules under prebiotic conditions.

[ 2 ]
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