IB Biology HL A3.1 Diversity of organisms Questions

Practise IB Biology HL A3.1 with classification and data questions on domains, kingdoms, shared characteristics and genetic, species and ecosystem biodiversity.

102 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic35 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts43
Paper types5

Most tested objectives

  1. A3.1.7—Karyotyping and karyograms9 papers
  2. A3.1.8—Unity and diversity of genomes within species6 papers
  3. A3.1.3—Binomial system4 papers
  4. A3.1.4—Biological species concept4 papers
  5. A3.1.2—Species as groups with shared traits3 papers

Common question formats

  • Data analysis
  • Definition or recall
  • Process explanation
  • Structured response
  • Diagram interpretation
  • Calculation
  • Comparison
  • Evaluation
  • Graph interpretation
  • Case study
  • Essay response
  • Extended response
  • Experimental design

Recent exam appearances

November 2025Paper 2 HL · TZ3Q7(c)[ 1 ]
November 2025Paper 2 HL · TZ1Q7(c)[ 4 ]
May 2025Paper 1B HL · TZ3Q1(a), 1(c)[ 5 ]

117 papers analysed · Tagging incomplete

Exam points

  • Classify organisms using shared morphological or population features, binomial naming, shared genus and dichotomous keys or character matrices.
  • Decide species membership from interbreeding, fertile offspring, gene flow and genetic evidence, and evaluate limits caused by hybrids, fossils, isolation or asexual and horizontal gene transfer.
  • Interpret ploidy, chromosome-number change, fusion, fission and polyploidy, and construct or analyse karyograms using chromosome structure, sex and abnormalities.
  • Explain shared and variable traits using genes, chromosomes, genome sequences, haplotypes and SNPs, including mutation, duplication and divergence of genome regions.
  • Compare genome sizes and gene numbers, explain why genome size need not track complexity, and evaluate model-organism choices for whole-genome sequencing.
  • Identify environmental DNA analysis as an application of DNA barcoding for detecting species.

Exam points

  • Group species using shared morphological and structural traits.
  • Explain why the morphological species concept is useful in fieldwork, with fossils and for many plants.
  • Explain how sexual dimorphism and variation within a species can make morphological classification difficult.

Question 1

[Maximum number: 2]

The land snail Cepaea nemoralis is very common in North America and in Europe.

The base colour of its shell varies between brown, pink and yellow, and also in its intensity. Some shells are unbanded, but most show one to five bands of different width on top of the shell base colour.

Figure for Question 1 — IB Biology HL

C. nemoralis is a mollusc. Identify two external features that distinguish this snail from an arthropod.

Exam points

  • Describe binomial nomenclature as a universal, two-part Latin name for each species.
  • Apply the naming rules: capitalize the genus, write the species name in lowercase, and italicize or underline both.
  • State that species in the same genus share similar traits.

Question 1

[Maximum number: 3]

Species are groups of organisms that can potentially interbreed to produce fertile offspring.

Suggest advantages of the system for naming species that scientists use.

Exam points

  • Define a species using the ability to interbreed and produce fertile offspring.
  • Explain how gene flow occurs between members of the same species.
  • Explain limits of the biological species concept for extinct organisms, asexual species and distinct forms with gene flow between them.

Question 1

[Maximum number: 3]

More than 8 million different species are alive today but over the course of evolution, more than 4 billion may have existed.

Outline the criteria that should be used to assess whether a group of organisms is a species.

Exam points

  • Explain that speciation is gradual as isolated populations diverge.
  • Explain that populations may still be theoretically able to interbreed even though they cannot meet.
  • Relate gradual divergence and inability to meet to species boundaries that can be partly arbitrary.

Question 1

[Maximum number: 3]

Evolution Canyon is a steep sided rocky valley near Mount Carmel in Israel. There are populations of fruit flies (Drosophila melanogaster) living on the north facing and south facing slopes of the canyon. Fruit flies are sensitive to drought conditions and die if they become desiccated.

Twenty samples of fruit flies were collected from both sides of the canyon. They were subjected to the same conditions of drought stress and the life span (in hours) of the adult flies was measured.

Figure for Question 1 — IB Biology HL

Discuss whether the populations on the two slopes could evolve into two species.

Exam points

  • State that chromosome number is usually constant within a species but varies between species.
  • Explain that diploid chromosome numbers are even because chromosomes occur in homologous pairs.
  • Recall the chromosome numbers in the examples: fruit fly 8, rice 24, human 46, chimpanzee 48 and dog 78.

Question 1

[Maximum number: 1]

Chromosome numbers vary between species. Which statement refers to humans?

A

An egg cell has 22 autosomes.

B

A sperm cell has 23 autosomes.

C

An egg cell has two X chromosomes.

D

A zygote has two autosomes.

Exam points

  • Use a karyotype to identify chromosome number and structure in a cell.
  • Arrange chromosomes in a karyogram by length, banding pattern and centromere position.
  • Explain how human chromosome 2 supports fusion from two ancestral ape chromosomes.

Question 1

[Maximum number: 1]

The karyogram is taken from a primate.

Figure for Question 1 — IB Biology HL

All primates have the same sex determination chromosomes. From which primate is the karyogram?

A

Human female

B

Chimpanzee female

C

Human male

D

Chimpanzee male

Exam points

  • Define a genome as all the genetic information in an organism.
  • Explain that members of a species share most of their genome but differ at genetic variants.
  • Describe SNPs as common single-base differences that can be used as biological markers.

Question 1

[Maximum number: 2]

The DNA sequences found in human cell lines of various ABO phenotypes are remarkably similar. The blood group gene that codes for intron 6 of glycosyltransferase consists of a 1062 base pair nucleotide sequence.

The table shows the sequence differences in intron 6 of the major ABO alleles for this gene. The nucleotides that are not shown are identical in the three blood group alleles, while the bolded letters show differences with the blood group A allele sequence.

Table for Question 1 — IB Biology HL

Calculate the percentage difference in bases between the allele for blood group A and the allele for blood group B for intron 6 of this gene, showing your workings.

Exam points

  • Describe variation in genome size and base sequence among eukaryotes.
  • Compare within-species and between-species variation, noting that between-species variation is much larger.
  • State that not all DNA codes for proteins.

Question 1

[Maximum number: 7]

Analysis of the genome of primates reveals many DNA segments that have been duplicated. It is possible to deduce the stage in evolution at which segmental duplications (SDs) occurred by comparing human and other primate genomes. Human-specific SDs occurred after humans and chimpanzees diverged. Human-chimpanzee shared SDs occurred after the common human-chimpanzee ancestor diverged from gorillas and so on. It is possible to estimate the rates at which SDs occurred during evolution using knowledge of when the primates changed. In the chart the bars indicate numbers of shared SDs and the line shows estimates of the rate of SDs per millions of years (Myr).

Figure for Question 1 — IB Biology HL

Question (a)

(a)

State the number of SDs that can be found in humans but not in other primates.

[ 1 ]

Question (b)

(b)

State the rate of SDs after the divergence of macaques from the other primates, giving the units.

[ 1 ]

Question (c)

(c)

Outline the trends in the rate of SDs occurring since the separation of orangutans from other primates.

[ 2 ]

Question (d)

(d)

Explain how the number of human-chimpanzee shared SDs can be the lowest despite the rate of SDs per millions of years being the highest.

[ 2 ]

Question (e)

(e)

A few SDs were found in humans and gorillas but not chimpanzees. Suggest how this might have occurred.

[ 1 ]

Exam points

  • Define genome size as the amount of DNA in one chromosome set.
  • Explain that a larger genome size does not necessarily mean greater organism complexity.
  • Explain how polyploidy can greatly increase genome size, especially in plants.

Question 1

[Maximum number: 1]

The table shows the estimated total number of genes in several organisms.

Table for Question 1 — IB Biology HL

What can be deduced from the information in this table?

A

Throughout evolution, the number of genes increases.

B

The domestic dog is more closely genetically related to the fruit fly than to the human.

C

The number of genes does not determine evolutionary success.

D

Humans produce about half as many proteins as rice.

Exam points

  • Describe how whole genome sequencing began at scale with the Human Genome Project.
  • Give current uses of whole genome sequencing: evolutionary relationships, gene discovery and pathogen research.
  • Describe future uses including personalized medicine, diagnosis, disease prevention and vaccine targets.

Question 1

[Maximum number: 3]

A protein expressed by the gene carotene desaturase (CTP1) was introduced into Golden rice (Oryza sativa) through genetic engineering. Bioinformatics were used to determine if the protein sequence matched any allergenic proteins (proteins that trigger an allergic reaction). The table shows the results from the alignment of the CTP1 protein with proteins from moth, soybean and dust mite that are known to be allergenic.

Table for Question 1 — IB Biology HL

Genes such as the one coding for CTP1 can be located by searching for open reading frames. Outline how open reading frames are identified.

Exam points

  • Explain why the biological species concept does not apply well to asexual organisms.
  • Explain how mutation and horizontal gene transfer create diversity in bacteria.
  • Explain how transformation, plasmids and recombination blur bacterial species boundaries.

Question 1

[Maximum number: 1]

The gut of a healthy human contains more than 1000 species of bacteria. Some of these species are shown in the cladogram.

Figure for Question 1 — IB Biology HL

Outline a difficulty in applying the biological species concept to bacteria.

Exam points

  • State that diploid and haploid chromosome numbers are usually constant within a species.
  • Explain how meiosis conserves chromosome number across generations.
  • Explain how different chromosome numbers can prevent fertile hybrids, as in mules.

Question 1

[Maximum number: 1]

Which is a characteristic of the haploid number of eukaryotic chromosomes?

A

It doubles in mitosis.

B

It is fixed for each species.

C

It is an even number for all species.

D

It is positively correlated with an animal's mass.

Exam points

  • Identify organisms by following the paired yes/no choices in a dichotomous key.
  • Construct a dichotomous key from observable features arranged in a character matrix.
  • Build and test a dichotomous key using local plant or animal species.

Question 1

[Maximum number: 1]

The following shows a dichotomous key.

Figure for Question 1 — IB Biology HL
Table for Question 1 — IB Biology HL

Using the dichotomous key above, identify the genus of bird W and bird Z .
Bird W:
Bird Z:

Exam points

  • Describe DNA barcoding as identifying species using short sequences from selected genes.
  • Explain how environmental DNA from habitats can be amplified by PCR and compared with databases.
  • Explain why DNA barcoding is rapid and useful for damaged, partial or hard-to-identify specimens.

Question 1

[Maximum number: 1]

What is one application of DNA barcoding?

A

Selective breeding

B

Preserving an endangered species

C

Analysing environmental DNA

D

Sequencing a genome