IB Biology HL B2.1 Membranes and membrane transport Questions

Practise IB Biology HL B2.1 with membrane diagrams and data questions covering selective permeability, transport mechanisms, fluidity, vesicles and ion pumps.

111 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic44 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts62
Paper types5

Most tested objectives

  1. B2.1.1—Lipid bilayers as basis7 papers
  2. B2.1.12 (HL)—Cholesterol and fluidity6 papers
  3. B2.1.15 (HL)—Sodium-potassium pumps6 papers
  4. B2.1.7—Pump proteins5 papers
  5. B2.1.10—Fluid mosaic model5 papers

Common question formats

  • Structured response
  • Comparison
  • Process explanation
  • Definition or recall
  • Diagram interpretation
  • Graph interpretation

Recent exam appearances

November 2025Paper 2 HL · TZ3Q3(b)[ 2 ]
November 2025Paper 1A HL · TZ3Q15[ 1 ]
November 2025Paper 1A HL · TZ1Q15[ 1 ]

117 papers analysed · Tagging incomplete

Exam points

  • Identify amphipathic phospholipid bilayers, explain head–tail orientation in water and relate the hydrophobic core to membrane barriers and compartment separation.
  • Define simple diffusion as passive net movement down a concentration gradient without ATP or transport proteins, including small non-polar molecules crossing the bilayer.
  • Distinguish integral, transmembrane and peripheral proteins and identify their channel, pump, receptor, enzyme, adhesion and recognition roles.
  • Define osmosis, predict water movement from solute or water-potential differences and explain how aquaporins increase water permeability.
  • Explain facilitated diffusion through selective channel proteins as passive movement down a gradient, using size, charge and polarity to explain selectivity.
  • Explain active transport by ATP-powered pumps, including sodium–potassium movement against gradients and the resulting ion gradients or resting potential.
  • Identify membrane glycoproteins and glycolipids with extracellular carbohydrate chains and relate the glycocalyx to recognition, adhesion and signalling.
  • Use the fluid mosaic model to locate hydrophilic heads, hydrophobic tails, embedded proteins and mobile membrane components, distinguishing it from the sandwich model.
  • Relate unsaturated and saturated fatty-acid composition and cholesterol to membrane packing, permeability, fluidity and temperature stability.
  • Explain how membrane fluidity permits vesicle budding, transport and fusion, and distinguish endocytosis from exocytosis.
  • Distinguish voltage-gated from ligand-gated ion channels and relate channel opening, membrane potential and potassium movement to nerve impulses.
  • Explain sodium-dependent glucose cotransport as secondary active transport driven by a sodium gradient maintained by basolateral pumps, including intestinal absorption.

Exam points

  • Identify phospholipids as amphipathic molecules with hydrophilic phosphate heads and hydrophobic fatty-acid tails.
  • Explain how phospholipids form a continuous, closed bilayer in water, with hydrophilic heads facing water and hydrophobic tails directed inwards.
  • Identify phospholipid bilayers in plasma membranes, vesicles and nuclear envelopes, and distinguish them from a nucleoid.

Question 1

[Maximum number: 1]

The diagram shows the original drawing of the cell membrane made in 1972 by Singer and Nicolson.

Figure for Question 1 — IB Biology HL

Label with the letter H part of a phospholipid molecule that is hydrophilic.

Exam points

  • Explain why polar, hydrophilic molecules such as glucose do not diffuse through the hydrophobic core of a phospholipid bilayer.
  • Use membrane examples to identify bilayers as barriers between aqueous compartments and recognise membrane-associated protein channels as routes for hydrophilic substances.

Question 1

[Maximum number: 1]

Glucose is absorbed through protein channels in the plasma membrane of epithelium cells in the small intestine. Which characteristics of glucose prevent its diffusion through the phospholipid bilayer?

A

It is non-polar and therefore hydrophobic.

B

Its hydrogen bonds link with amino acids in the protein channel.

C

It is polar and therefore hydrophilic.

D

Its covalent bonds interact with the phospholipids.

Exam points

  • Define simple diffusion as passive net movement of particles down a concentration gradient using random kinetic motion, without ATP or transport proteins.
  • Explain how a steeper concentration gradient increases the rate of simple diffusion until net movement stops at equilibrium.
  • Identify small non-polar molecules such as oxygen and carbon dioxide that cross directly through the phospholipid bilayer by simple diffusion.

Question 1

[Maximum number: 1]

Which process results in the exchange of gases across the membrane of pneumocytes?

A

Active transport

B

Simple diffusion

C

Facilitated diffusion

D

Mass flow

Exam points

  • Distinguish integral proteins embedded in one or both bilayer layers from peripheral proteins attached to a surface.
  • Match membrane proteins to functions: channels, pumps, receptors, enzymes, adhesion or cell recognition.
  • Draw a membrane diagram placing integral, peripheral and glycoprotein components relative to the bilayer.

Question 1

[Maximum number: 1]

How is facilitated diffusion in axons similar to active transport?

A

They both require the energy of ATP.

B

They both move substances against a concentration gradient.

C

They both use sodium-potassium pumps.

D

They are both carried out by proteins embedded in the axon membrane.

Exam points

  • Define osmosis as passive water movement across a partially permeable membrane from lower to higher solute concentration.
  • Predict net water movement in hypertonic or hypotonic cell examples, including across frog-egg membranes.
  • Use aquaporin mRNA/control graphs to explain faster water uptake, cell swelling and bursting.

Question 1

[Maximum number: 3]

In the early 1990s, investigations involving egg cells from the African clawed frog (Xenopus laevis) led to the discovery of membrane channel proteins called aquaporins.

In one investigation, scientists injected 10 frog egg cells with a solution containing mRNA encoding for an aquaporin, and another 10 frog egg cells with distilled water (control cells). All the frog egg cells were then incubated in a hypotonic solution. The volume of the frog egg cells was then measured during a period of five minutes.

The graph shows the mean results of the investigation.

Figure for Question 1 — IB Biology HL

Question (a)

(a)

State the direction of net movement of water across the plasma membrane of all the frog egg cells between 0 and 3 minutes.

[ 1 ]

Question (b)

(b)

Explain the reasons for the different results obtained for the frog egg cells with aquaporin mRNA and the control frog egg cells.

[ 2 ]

Exam points

  • Explain why polar amino acids line channels that carry ions or hydrophilic solutes.
  • Describe channel-mediated diffusion as selective, passive movement down a gradient without ATP, ending at equilibrium.
  • Interpret chloride-concentration graphs showing facilitated-diffusion rate rising then levelling off.

Question 1

[Maximum number: 1]

By which process do potassium ions move through potassium channels in axons?

A

Active transport

B

Exocytosis

C

Facilitated diffusion

D

Simple diffusion

Exam points

  • Explain active transport as ATP-powered movement of specific ions against concentration gradients.
  • Use the Na+/K+ pump diagram to trace ATP-driven Na+ export and K+ uptake against gradients.
  • Interpret intracellular/extracellular Na+ and K+ concentration tables maintained by pump proteins.

Question 1

[Maximum number: 1]

A cell was placed into a solution containing a dye. After two hours the concentration of the dye inside the cell was higher than in the solution. This was repeated in the presence of a substance that inhibits ATP. In this case the dye did not enter the cell. What is the probable mechanism by which the dye entered the cell?

A

Active transport

B

Simple diffusion

C

Osmosis

D

Facilitated diffusion

Exam points

  • Choose simple diffusion for small, non-polar solutes or facilitated diffusion for larger or polar solutes.
  • Compare both routes as passive, down-gradient transport, but only facilitated diffusion uses selective proteins.
  • Match oestradiol with the bilayer and fructose with facilitated diffusion in molecule-route tables.
  • Relate facilitated-diffusion rate to transport-protein abundance when comparing it with simple diffusion.

Question 1

[Maximum number: 2]

The diagram shows a cell from the nervous system.

Figure for Question 1 — IB Biology HL

Compare and contrast the movement of particles across plasma membranes by simple diffusion and facilitated diffusion.

Exam points

  • Identify glycoproteins and glycolipids by their short carbohydrate chains on the extracellular surface of the membrane.
  • Explain how the glycocalyx supports cell recognition, adhesion, signalling and interactions with water.

Exam points

  • Label a fluid mosaic membrane with hydrophilic heads, hydrophobic tails, integral proteins, peripheral proteins and other embedded components.
  • Explain fluidity as movement of phospholipids and proteins within the bilayer, with proteins and other molecules distributed in a mosaic.
  • Use evidence for integral and peripheral protein placement to distinguish the Singer–Nicolson model from the Davson–Danielli sandwich model.
  • Identify freeze-fracture and etching as techniques used to produce membrane electron micrographs.

Question 1

[Maximum number: 1]

The drawings are from two original papers that proposed a structure of the cell membrane.

For what reason is the Singer-Nicolson model considered a better representation of the cell membrane than the Davson-Danielli model?

A

It has extrinsic proteins.

B

It shows how the phospholipid bilayer is arranged.

C

It helps in the understanding of the fluidity of the cell membrane.

D

It shows how oxygen can diffuse into the cell.

Exam points

  • Relate a higher proportion of unsaturated fatty-acid tails to kinks, looser packing and greater membrane fluidity.
  • Relate a higher proportion of saturated fatty-acid tails to closer packing and reduced membrane fluidity.
  • Predict fatty-acid composition changes needed to maintain membrane fluidity when temperature changes.

Question 1

[Maximum number: 1]

Which changes would take place in the plasma membrane composition to maintain fluidity at very low temperatures?

Unsaturated fatty acids

Saturated fatty acids

Proteins

increase

increase

decrease

no change

increase

decrease

increase

decrease

no change

decrease

no change

increase

Exam points

  • Identify cholesterol as a membrane component that regulates membrane fluidity.
  • Explain how cholesterol prevents membranes becoming too fluid at high temperatures and stiffening or solidifying at low temperatures.
  • State that cholesterol reduces membrane permeability to some substances.

Question 1

[Maximum number: 1]

What is a function of cholesterol in the cell membrane?

A

To regulate fluidity

B

To store energy

C

To provide a channel for transport

D

To hold adjacent cells together

Exam points

  • Explain how membrane fluidity allows vesicles to bud from and fuse with membranes.
  • Describe endocytosis as membrane invagination around material followed by pinching off to form an internal vesicle.
  • Explain exocytosis as vesicle fusion with the plasma membrane to release material, and describe vesicle transport between cell compartments.

Question 1

[Maximum number: 5]

Describe the process of endocytosis.

Exam points

  • Distinguish voltage-gated and neurotransmitter/ligand-gated ion channels as selective pores that open and close.
  • Relate membrane potential or neurotransmitter binding to opening and closing of gated ion channels.
  • Explain potassium-ion movement through voltage-gated channels during repolarization of a nerve impulse.

Question 1

[Maximum number: 1]

The table shows two types of gated ion channels in neurons. How are they gated?

Nicotinic acetylcholine
receptors

Potassium channels

voltage

neurotransmitter

neurotransmitter

voltage

voltage

voltage

neurotransmitter

neurotransmitter

Exam points

  • Explain that ATP-powered sodium-potassium pumps move three sodium ions out of a cell and two potassium ions into it per cycle.
  • Relate sodium-potassium pump activity to resting potential and its restoration after stimulation.
  • Interpret experimental data on how hypoxia and nitric oxide synthase inhibition affect sodium-potassium pump concentration.

Question 1

[Maximum number: 1]

The table shows concentrations of potassium ions and sodium ions inside and outside human cells.

Table for Question 1 — IB Biology HL

What explains these concentrations?

A

Potassium ions diffuse in and sodium ions diffuse out.

B

Sodium ions diffuse in and potassium ions diffuse out.

C

Active transport pumps sodium ions in and potassium ions out.

D

Active transport pumps sodium ions out and potassium ions in.

Exam points

  • Identify sodium-dependent glucose cotransport as indirect active transport into epithelial cells.
  • Relate the sodium gradient maintained by basolateral pumps to glucose uptake in the ileum or nephron.
  • Use ileal absorption as an example of sodium-coupled nutrient uptake by epithelial cells followed by transport into nearby capillaries.

Question 1

[Maximum number: 6]

Explain the mechanisms used by the ileum to absorb and transport food.

No public sample questions are available for this syllabus point yet.

Question 1

[Maximum number: 5]

The diagram shows the original drawing of the cell membrane made in 1972 by Singer and Nicolson.

Figure for Question 1 — IB Biology HL

Question (a)

(a)

Label with the letter H part of a phospholipid molecule that is hydrophilic.

[ 1 ]

Question (b)

(b)

Distinguish between this model of the cell membrane and the Davson-Danielli model.

[ 1 ]

Question (c)

(c)

Explain the role of molecules such as M in sodium-potassium pumps.

[ 3 ]
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