IB Biology HL B2.2 Organelles and compartmentalization Questions

Practise IB Biology HL B2.2 by linking compartmentalization, nuclear gene expression and protein pathways to organelle structures for respiration and photosynthesis.

53 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic27 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts30
Paper types5

Most tested objectives

  1. B2.2.7 (HL)—Free ribosomes vs. rough ER9 papers
  2. B2.2.4 (HL)—Mitochondrion adaptations5 papers
  3. B2.2.5 (HL)—Chloroplast adaptations5 papers
  4. B2.2.8 (HL)—Golgi apparatus3 papers
  5. B2.2.9 (HL)—Vesicles in cells2 papers

Common question formats

  • Definition or recall
  • Diagram interpretation
  • Process explanation
  • Structured response
  • Extended response

Recent exam appearances

November 2025Paper 1A HL · TZ3Q16[ 1 ]
November 2025Paper 1A HL · TZ1Q16[ 1 ]
May 2025Paper 2 HL · TZ3Q9(a)[ 4 ]

117 papers analysed · Tagging incomplete

Exam points

  • Identify the nucleus and membrane-bound organelles as discrete eukaryotic structures, distinguishing them from prokaryotic cell components.
  • Distinguish transcription in the nucleus from translation in the cytoplasm, including each process’s product and machinery.
  • Explain how compartmentalization concentrates enzymes and substrates, provides suitable local pH, separates incompatible processes and confines acidic digestion in lysosomes.
  • Relate mitochondrial cristae surface area, the intermembrane proton gradient and matrix enzymes to respiration and ATP production.
  • Identify grana, thylakoids and stroma in chloroplasts and relate thylakoid proton accumulation and stroma enzymes to photosynthesis.
  • Explain the benefit of the double nuclear envelope, including its breakdown into vesicles during mitosis.
  • Distinguish free from rough-ER-bound ribosomes by protein destination, and identify polysomes when interpreting cell diagrams.
  • Trace protein processing from rough ER to Golgi modification, sorting and vesicle delivery to lysosomes, membranes or secretion.
  • Use vesicle diagrams to identify exocytosis of enzyme cargo and explain how clathrin helps form coated vesicles by indenting the membrane.

Exam points

  • Identify membrane-bound organelles such as the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes as discrete eukaryotic subunits.
  • Distinguish eukaryotic organelles and linear chromosomes from the structures present in prokaryotic cells.

Question 1

[Maximum number: 4]

Testis cells are eukaryotic cells. Identify the structures seen under the electron microscope in testis cells that are not present in prokaryotic cells.

Exam points

  • Distinguish transcription in the nucleus from translation in the cytoplasm, including their different products and molecular machinery.

No public sample questions are available for this syllabus point yet.

Exam points

  • Explain how compartments separate incompatible reactions and keep lysosomal hydrolases away from cytoplasm.
  • Explain how organelles concentrate enzymes and substrates at a pH suited to each reaction.
  • Relate small compartment volumes to rapid proton or concentration gradient formation.

Question 1

[Maximum number: 4]

The structure of membranes allows them to carry out many functions in cells.

Discuss the use of membranes for compartmentalization in eukaryotic cells.

Exam points

  • Explain how cristae increase inner-membrane surface area for electron transport and ATP synthesis.
  • Relate the narrow intermembrane space to proton gradients and the matrix to Krebs-cycle enzymes.

Question 1

[Maximum number: 1]

Eukaryotic cells have membrane-bound organelles. What is a property of one of these organelles?

A

The rough endoplasmic reticulum synthesises lipids for use outside the cell.

B

The nucleus has a double membrane to allow vesicle formation for intracellular transport.

C

The mitochondrion has enzymes and substrates of the Krebs cycle compartmentalized in the matrix.

D

The Golgi apparatus uses ribosomes to synthesise proteins for export from the cell.

Exam points

  • Identify chloroplast thylakoids, grana and stroma in diagrams or micrographs.
  • Link grana surface area and photosystems to electron transport, and small thylakoid spaces to proton gradients.
  • Relate stroma enzymes and suitable conditions to carbon fixation and the Calvin/light-independent reactions.

Question 1

[Maximum number: 4]

Describe how the structure of the chloroplast is adapted to its function in photosynthesis.

Exam points

  • Identify vesicle breakdown during mitosis as a benefit of the double nuclear envelope.

Question 1

[Maximum number: 1]

What is a benefit of double membranes surrounding the nuclei of eukaryotic cells?

A

They reduce the surface area of the nucleus.

B

They can easily break down into vesicles during mitosis.

C

Hydrophilic phospholipid tails can be kept away from the cytoplasm.

D

Pores are formed to allow movement of DNA from the nucleus to the cytoplasm.

Exam points

  • Compare cytoplasmic proteins from free ribosomes with ER-bound proteins for secretion or lysosomes.
  • Label rough endoplasmic reticulum and polysomes on an electron micrograph of a cell.

Question 1

[Maximum number: 1]

Which statement distinguishes between the roles of free and bound ribosomes?

A

Free ribosomes synthesize proteins for use inside the cell, whereas bound ribosomes synthesize proteins mainly for export.

B

Free ribosomes synthesize proteins mainly for export, whereas bound ribosomes synthesize proteins for use inside the cell.

C

Free ribosomes synthesize proteins, whereas bound ribosomes do not.

D

Bound ribosomes synthesize proteins, whereas free ribosomes do not.

Exam points

  • Identify the Golgi apparatus in cell diagrams or electron micrographs.
  • Explain Golgi modification and vesicle delivery of RER proteins to secretion, membranes or lysosomes.

Question 1

[Maximum number: 3]

Outline the functions of rough endoplasmic reticulum and Golgi apparatus.

Exam points

  • Explain how exocytosis releases enzyme cargo from pancreatic exocrine cells.
  • Explain how clathrin binding indents the membrane during vesicle formation.

Question 1

[Maximum number: 1]

The image shows an electron micrograph of pancreatic exocrine cells.

Figure for Question 1 — IB Biology HL

What is the role of the vesicles shown in the micrograph?

A

To transport hormones between the rough endoplasmic reticulum and the Golgi apparatus

B

To store glycogen when blood glucose levels are high

C

To move enzymes out of the cell by exocytosis

D

To digest cellulose