IB Biology HL C2.2 Neural signalling Questions

Practise IB Biology HL C2.2 with membrane-potential traces and synapse data covering action potentials, neurotransmitters, saltatory conduction, summation and drugs.

130 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic62 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts86
Paper types5

Most tested objectives

  1. C2.2.12 (HL)—Exogenous chemicals22 papers
  2. C2.2.8 (HL)—Depolarization and repolarization10 papers
  3. C2.2.6—Neurotransmitter release9 papers
  4. C2.2.1—Neurons6 papers
  5. C2.2.11 (HL)—Saltatory conduction6 papers

Common question formats

  • Calculation
  • Comparison
  • Data analysis
  • Diagram interpretation
  • Process explanation
  • Structured response
  • Definition or recall

Recent exam appearances

November 2025Paper 2 HL · TZ3Q3(a), 3(c)[ 5 ]
November 2025Paper 2 HL · TZ1Q8(b)[ 7 ]
November 2025Paper 1B HL · TZ1Q3(a), 3(c)(i), 3(c)(ii), 3(d)[ 6 ]

117 papers analysed · Tagging incomplete

Exam points

  • Label neuron structures and distinguish motor and sensory neuron arrangements and connections.
  • Define resting potential and explain how the ATP-driven sodium-potassium pump maintains 3 Na⁺-out/2 K⁺-in gradients and axon polarization.
  • Explain action-potential initiation through threshold and all-or-none response, voltage-gated Na⁺ influx/depolarization, delayed K⁺ efflux and repolarization or hyperpolarization.
  • Interpret action-potential traces and oscilloscope output using threshold, ion movement and channel timing, including membrane-potential change calculations.
  • Calculate and compare conduction speeds using axon-diameter and myelin data.
  • Explain how local Na⁺ currents depolarize the next axon region to threshold during propagation.
  • Identify nodes of Ranvier and myelin, then explain node-to-node saltatory conduction and speed.
  • Explain one-way synaptic transmission using acetylcholine receptor binding, breakdown and postsynaptic effects.
  • Explain how an arriving action potential opens Ca²⁺ channels, fuses vesicles and releases neurotransmitter.
  • Explain EPSP receptor binding and postsynaptic ion flow that causes depolarization.
  • Explain how exogenous chemicals alter synaptic transmission by mimicking, blocking or prolonging neurotransmitter effects.
  • Distinguish excitatory and inhibitory inputs and explain temporal/spatial summation reaching threshold.
  • Interpret fMRI scans to compare activation patterns and motor function after stroke.

Exam points

  • Label a motor neuron’s cell body and nucleus, dendrites, axon, myelin sheath, nodes of Ranvier and motor end plates.
  • Name the sensory, relay and motor neurons in a reflex arc, and identify that a motor neuron’s cell body lies in the CNS.

Question 1

[Maximum number: 2]

Young and old rabbits were tested in a classic reflex conditioning experiment. The ringing of a bell (conditioned stimulus) was followed after a delay of either 600 or 750 milliseconds (ms) by an eyeblink stimulus consisting of a mild puff of air to the cornea (unconditioned stimulus). Conditioned learning was measured by the percentage of rabbits that blinked their eyes with the ringing of the bell only. The graph shows the outcome after several trials.

Figure for Question 1 — IB Biology HL

Question (a)

(a)

The diagram shows a reflex arc.

Figure for Question (a) — IB Biology HL

On the diagram, label

[ 2 ]

Question (i)

(i)

On the reflex arc diagram, label a motor neuron.

[ 1 ]

Question (ii)

(ii)

On the reflex arc diagram, label a sensory neuron.

[ 1 ]

Exam points

  • Define resting potential and state its approximate membrane-potential value (about −70 mV).
  • Explain how the sodium-potassium pump uses ATP to move 3 Na⁺ out and 2 K⁺ in, maintaining axon ion gradients and polarization.

Question 1

[Maximum number: 1]

What occurs during the establishment of a resting membrane potential of a neuron?

A

Both sodium and potassium ions are pumped outside the neuron.

B

Sodium ions are pumped out while potassium ions are pumped into the neuron.

C

Both sodium and potassium ions are at rest inside the membrane of the neuron.

D

Sodium ions leave by diffusion and potassium ions enter the neuron by active transport.

Exam points

  • Explain how stimulus-triggered sodium influx depolarizes and reverses membrane polarity, followed by potassium efflux and repolarization.
  • Describe how threshold depolarization initiates an action potential that propagates to adjacent membrane along a nerve fibre.

Question 1

[Maximum number: 1]

Where could an action potential occur in a motor neuron?

A

Anywhere on the cell membrane

B

Dendrites only

C

Cell body only

D

Axon only

Exam points

  • Calculate and compare conduction velocities using experimental nerve-fibre data.
  • Relate larger axon diameter and a thicker myelin sheath to faster nerve impulse conduction.

Question 1

[Maximum number: 1]

The conduction velocity of electrical impulses was measured in different myelinated nerve fibres.

Table for Question 1 — IB Biology HL

Which hypothesis would explain the higher conduction velocity of nerve fibre R, compared to nerve fibres P and Q ?

A

It is the longest.

B

It has the thinnest myelin sheath.

C

It has the largest diameter.

D

It has the greatest number of mitochondria.

Exam points

  • Explain one-way chemical synaptic transmission from presynaptic calcium entry and vesicle release to postsynaptic receptor activation.
  • Identify the fate of acetylcholine after receptor binding, including its breakdown in the synaptic cleft.
  • Identify the synaptic junction structure shown in a neuron diagram.
  • Label a neurotransmitter receptor on a synapse diagram.

Question 1

[Maximum number: 1]

The diagram shows the synaptic transmission of nerve impulses by the neurotransmitter acetylcholine.

Figure for Question 1 — IB Biology HL

What is the fate of acetylcholine immediately after binding to the receptor?

A

It is pumped into the postsynaptic neuron.

B

It diffuses into the presynaptic neuron.

C

It is broken down in the synaptic cleft.

D

It binds to another receptor in the postsynaptic neuron.

Exam points

  • Explain how an arriving action potential depolarizes the presynaptic terminal, opens voltage-gated Ca²⁺ channels and triggers Ca²⁺ influx, vesicle fusion and neurotransmitter exocytosis.

Question 1

[Maximum number: 1]

What occurs during the transmission of an impulse at a synapse?

I. Acetylcholine is released into the synaptic cleft by exocytosis when calcium ions enter the presynaptic neuron.
II. Acetylcholine diffuses across the synaptic cleft and binds to receptors on the postsynaptic membrane.
III. Acetylcholine is broken down by cholinesterase and resorbed into the presynaptic neuron once an impulse has been initiated on the postsynaptic membrane.

A

I and II only

B

II and III only

C

I and III only

D

I, II and III

Exam points

  • Explain how neurotransmitter binding at a postsynaptic receptor changes ion flow and depolarizes the membrane, potentially initiating an action potential.

Question 1

[Maximum number: 1]

Atropine drops are used by opticians to dilate the pupil, so that a thorough examination of the retina can be performed. Atropine binds to acetylcholine receptors in synapses.

Figure for Question 1 — IB Biology HL
Figure for Question 1 — IB Biology HL

What is the effect of atropine binding in synapses?

A

Inhibits the binding of acetylcholine at the presynaptic membrane

B

Inhibits the release of acetylcholine from the presynaptic neuron

C

Prevents binding of acetylcholine at the postsynaptic membrane

D

Prevents transport of acetylcholine through the postsynaptic membrane

Exam points

  • Explain how reaching threshold opens voltage-gated Na⁺ channels; Na⁺ influx depolarizes the axon membrane.
  • Explain how voltage-gated K⁺ channels open after depolarization; K⁺ efflux repolarizes the membrane and may briefly hyperpolarize it when channels close slowly.

Question 1

[Maximum number: 1]

The graph below shows an action potential.

Figure for Question 1 — IB Biology HL

Where on the graph do the sodium channels open?

A

At 1

B

Between 1 and 2

C

At 2

D

Between 2 and 3

Exam points

  • Explain how local currents from sodium-ion diffusion depolarize the next region of an axon to threshold during action-potential propagation.

Question 1

[Maximum number: 3]

The image shows human red blood cells.

Figure for Question 1 — IB Biology HL

Explain the propagation of nerve impulses along the membrane of a neuron.

Question 1

[Maximum number: 1]

The graph shows an action potential.

Figure for Question 1 — IB Biology HL

What is the change in membrane potential from resting potential to complete depolarization?

A

-70 mV

B

15 mV

C

40 mV

D

110 mV

Exam points

  • Explain how myelin insulates an axon so action potentials regenerate at nodes of Ranvier, jumping from node to node and increasing conduction speed.

Question 1

[Maximum number: 1]

The image shows a neuron.

Figure for Question 1 — IB Biology HL

What is the function of X ?

A

Increases the speed of transmission along the axon

B

Increases the rate of exchange of sodium and potassium ions

C

Holds bundles of neurons together to form a nerve

D

Determines the direction of the action potential

Exam points

  • Explain how neonicotinoids act like acetylcholine, bind insect postsynaptic receptors, resist breakdown and disrupt synaptic transmission.
  • Explain how cocaine blocks presynaptic dopamine reuptake, causing dopamine to accumulate in the cleft and prolong postsynaptic stimulation.

Question 1

[Maximum number: 1]

Diagrams X and Y show the possible action on synaptic transmission of two exogenous chemicals.

Figure for Question 1 — IB Biology HL

Key:
- neurotransmitter
- neonicotinoid or cocaine
Cocaine and neonicotinoids have different modes of action. Which statement identifies the correct mode of action of one of these chemicals?

A

Y shows cocaine because it prevents binding of the neurotransmitter to the postsynaptic membrane.

B

X shows neonicotinoid because it is taken up by the presynaptic terminal.

C

Y shows neonicotinoid because it binds to the neurotransmitter.

D

X shows cocaine because the neurotransmitter cannot be taken up by the presynaptic terminal.

No public sample questions are available for this syllabus point yet.

Exam points

  • Explain how excitatory inputs depolarize a postsynaptic neuron while inhibitory inputs hyperpolarize it, and how their combined effects determine whether threshold is reached.

Question 1

[Maximum number: 2]

An organism's diminished response to a repeated stimulus is referred to as habituation. The diagrams show the experiments performed on habituation to a stimulus in a sea slug (Aplysia californica). The sea slug is a mollusc that has an external gill for gas exchange. If the siphon of the sea slug is stimulated mechanically, it withdraws the gill in a simple reflex action.

Control

Control

Control

Control

With repeated stimulus (habituation), the sea slug no longer withdraws the gill.

Habituated

Habituated

With reference to the withdrawal of the siphon in Aplysia californica, explain the concept of summation.

No public sample questions are available for this syllabus point yet.

Exam points

  • Identify fMRI as a method for functional brain analysis and compare activation patterns across brain regions or groups to infer their association with a function.

Question 1

[Maximum number: 2]

Compare and contrast the scan results for the healthy controls with the scans for the stroke survivors.