IB Biology HL D4.1 Natural selection Questions

Practise IB Biology HL D4.1 by interpreting selection evidence, tracing allele-frequency change and applying Hardy-Weinberg calculations to populations.

120 questions in this syllabus scope, with question parts counted together.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam analysis

HL · 2010–2025
Papers with this topic57 / 117in the analysed sample
Latest appearanceNovember 2025
Tagged question parts74
Paper types5

Most tested objectives

  1. D4.1.1—Natural selection as mechanism15 papers
  2. D4.1.12 (HL)—Types of selection14 papers
  3. D4.1.13 (HL)—Hardy-Weinberg equation8 papers
  4. D4.1.14 (HL)—Hardy-Weinberg conditions7 papers
  5. D4.1.7—Sexual selection6 papers

Common question formats

  • Process explanation
  • Data analysis
  • Definition or recall
  • Calculation
  • Diagram interpretation
  • Experimental design
  • Comparison

Recent exam appearances

November 2025Paper 1B HL · TZ3Q4(d)[ 3 ]
November 2025Paper 1B HL · TZ1Q4(c), 4(d)[ 6 ]
November 2025Paper 1A HL · TZ3Q38[ 1 ]

117 papers analysed · Tagging incomplete

Exam points

  • Explain how heritable variation and selection shift survival, reproduction and trait frequencies.
  • Explain how mutation creates new alleles, while meiosis and fertilisation create new combinations.
  • Interpret freezing-exposure data to evaluate selection for an inherited trait.
  • Explain how mate choice for courtship traits changes reproductive success.
  • Analyse Endler guppy data to distinguish predator and mate-choice effects on colour.
  • Define a gene pool and explain how isolation can drive allele-frequency divergence.
  • Explain how selection, mutation and recombination shift allele frequencies over generations.
  • Distinguish directional, stabilizing and disruptive selection from phenotype distributions.
  • Calculate allele, genotype or phenotype frequencies using p + q = 1 and p² + 2pq + q² = 1.
  • State Hardy-Weinberg assumptions and identify evolutionary forces that disrupt equilibrium.

Exam points

  • Explain how heritable variation and selection pressure alter survival, reproduction and trait frequency.
  • Use predation, disease or environmental change to explain which inherited trait spreads in a population.

Question 1

[Maximum number: 2]

The willie wagtail (Rhipidura leucophrys) is a common species of bird found in New Guinea and Australia. It feeds by foraging for a diverse selection of prey, including arthropods and lizards. When a willie wagtail catches prey, it either eats it or flies back to the nest to feed a young nestling bird. Researchers studied the feces of the birds to determine the size of prey eaten by the adults and the nestlings. The results are shown in the chart.

Figure for Question 1 — IB Biology HL

Explain how the foraging behaviour shown in the graph could have evolved in willie wagtails.

Exam points

  • Explain how inherited germ-line mutations create new alleles, while meiosis and random fertilisation produce new combinations of existing alleles.

Question 1

[Maximum number: 1]

What would restrict evolution by natural selection, if a species only reproduced by cloning?

A

Too few offspring would be produced.

B

Mutations could not occur.

C

The offspring would show a lack of variation.

D

The offspring would be the same sex as the parent.

Exam points

  • Identify overproduction and competition as conditions that promote natural selection.

Question 1

[Maximum number: 1]

What promotes natural selection?

I. Overpopulation
II. Competition
III. Variation

A

I and II only

B

I and III only

C

II and III only

D

I, II and III

Exam points

  • Evaluate population data to assess whether freezing exposure could select for differences in an inherited trait.

Question 1

[Maximum number: 2]

White clover (Trifolium repens) is native to Eurasia but is now a common plant found worldwide in lawns, next to roads, in pastures and similar habitats.

Figure for Question 1 — IB Biology HL

Some T. repens plants are able to produce the toxin hydrogen cyanide (HCN) by cyanogenesis. A study at 128 sites ( 2509 plants) in Toronto (Canada) looked at the proportion of T. repens plants producing HCN. The sites were at regular intervals from the city centre towards rural areas.

Figure for Question 1 — IB Biology HL

The researchers then investigated a possible correlation between cyanogenesis and exposure to freezing conditions. It had been proposed that when a cyanogenic plant freezes, its cells burst, releasing HCN which is toxic to the plant. Snow can insulate the ground and plants from freezing temperatures. However, snow is more likely to melt in cities, which then exposes plants to freezing temperatures.

All four of the cities studied receive below freezing temperatures and winter snowfall. Researchers looked at the number of days below freezing (0∘C)\left(0^{\circ} \mathrm{C}\right) that did not have snow cover in these cities.

Figure for Question 1 — IB Biology HL

Using all of the data so far, suggest whether exposure to freezing temperatures in the four cities is supported as a reason for the differences in HCN production in T. repens.

Exam points

  • Explain biological fitness by linking an adaptive trait to higher survival or reproductive success and transmission of its alleles to offspring in that environment.

Question 1

[Maximum number: 4]

Mandible (mouthpart) size is variable in the male broadhorned flour beetle (Gnatocerus cornutus).

Figure for Question 1 — IB Biology HL

In a laboratory experiment, scientists investigated how natural selection by predation on flour beetles by the larger assassin bug (Amphibolus venator) affected male mandible size. The graph shows the mean and standard deviation (SD) male mandible size over seven generations in three replicate experiments. Control populations were not subjected to predation.

Figure for Question 1 — IB Biology HL

Explain how natural selection by predation affects male mandible size in flour beetles.

Exam points

  • Evaluate mate-choice data by considering trends, scatter and sample limitations before concluding whether an ornament influences female preference.
  • Explain how courtship, displays or competitive traits can improve mating success and reproductive success through sexual selection.

Question 1

[Maximum number: 1]

Coho salmon, Oncorhynchus kisutch, displays two distinct male phenotypes commonly referred to as "jack" and "hooknose". Females will spawn with both male phenotypes. Hooknose males are larger, have greater fat reserves and have more strongly developed secondary sex characteristics.

jack male

jack male

hooknose male

hooknose male

The reproductive behaviour of the two phenotypes is different. Jack males prefer a less aggressive approach to females while hooknose males display a more threatening, aggressive approach.

In the presence of a male (hooknose or jack), females display digging and egg-laying behaviour. Digging involves making one or more holes in the riverbed to receive the eggs. The occurrence of digging behaviour and the time spent laying eggs were recorded over two mating seasons.

Figure for Question 1 — IB Biology HL
Figure for Question 1 — IB Biology HL

Suggest an evolutionary advantage for the presence of stable populations of jack and hooknose males in coho salmon populations.

Exam points

  • Interpret Endler guppy evidence by relating predator abundance to colour patterns or spot size.

Question 1

[Maximum number: 1]

John Endler experimented on populations of guppies (Poecilia reticulata) with different colouration. A male guppy fish is shown with large spots, which makes the fish more attractive to females, but more visible to predators.

Figure for Question 1 — IB Biology HL

The table shows the male colouration of guppy fish and number of predators in three different ponds.

Table for Question 1 — IB Biology HL

What can be concluded from the data?

A

There is a positive correlation between numbers of predator X and size of spots.

B

Predator Y has least influence on colouration.

C

There is a negative correlation between number of predators and size of spots.

D

There is no sexual selection.

Exam points

  • Define a gene pool as all genes and alleles in an interbreeding population at a given time.
  • Explain how isolation and selection can change allele frequencies within gene pools over generations.

Question 1

[Maximum number: 2]

Cystic fibrosis (CF) is caused by a mutation of a human gene which codes for a chloride channel. The frequency of the CF allele is much higher in Europe than expected for an allele that causes a harmful condition. It has been suggested that individuals who are heterozygous for this allele may be protected against an infectious disease such as cholera or typhoid. This could cause both the CF allele and the normal allele of the chloride channel gene to persist in the population.

Using CF as an example, distinguish between allele frequency and gene pool.

Exam points

  • Explain how selection raises an allele’s frequency when it improves survival or reproductive success.
  • Describe how mutation can produce a resistance allele that natural selection then favours.

Question 1

[Maximum number: 1]

The ability to digest lactose in adulthood appeared due to a mutation in the lactase gene. The frequency of the lactase persistence allele was recorded as 0.8 in present-day European populations and as 0.05 in fossils from populations of their prehistoric ancestors.

What could have caused the change in the allele frequency?

A

Drinking more milk caused the mutation to occur.

B

There was a strong positive selection for the lactase persistence allele.

C

Lactase persistence was transferred to humans from cows.

D

Prehistoric milk did not contain lactose.

Exam points

  • Identify directional, stabilizing and disruptive selection from the phenotype patterns and examples described in a question.
  • Explain which phenotypes a selection pattern favours or removes, such as one extreme, both extremes or intermediate forms.

Question 1

[Maximum number: 1]

What is an outcome of selection?

A

Directional selection increases the size of the gene pool.

B

Stabilizing selection increases allele frequencies of phenotypic extremes.

C

Disruptive selection increases genetic variance in a population.

D

Sexual selection leads to allopatric speciation.

Exam points

  • State and use p + q = 1 and p² + 2pq + q² = 1 to derive expected genotype frequencies in an equilibrium population.
  • Calculate expected genotype or phenotype frequencies and population counts from allele frequencies using q², p² and 2pq.

Question 1

[Maximum number: 1]

The Hardy-Weinberg equation, used to calculate expected genotype frequencies in a population in genetic equilibrium, is:

p2+2pq+q2=1p^{2}+2 p q+q^{2}=1

The length of the mouthparts in an insect population is controlled by a gene with two alleles: long mouthparts (dominant) and short mouthparts (recessive). The frequency of the allele for long mouthparts is 0.72 .

What is the expected genotype frequency of individuals with short mouthparts?

A

0.52

B

0.40

C

0.28

D

0.08

Exam points

  • Identify the Hardy-Weinberg equilibrium assumptions: a large population, random mating, and no selection, mutation, migration or genetic drift.
  • Recognize equilibrium through constant allele and genotype frequencies, and identify assumptions whose failure allows those frequencies to change.

Question 1

[Maximum number: 1]

Which of these conditions is/are necessary for genotype frequencies to fit the Hardy-Weinberg equation?

I. Homozygous recessive phenotypes have lower survival.
II. Mating is random.
III. The population is small.

A

I only

B

II only

C

II and III only

D

I, II and III

Exam points

  • Recognize selective breeding as artificial selection and give variation among dog breeds as a resulting phenotype.

Question 1

[Maximum number: 1]

Which is an example of evolution by selective breeding?

A

Selection of prey animals that can run faster than their predators

B

The variation in the size of different breeds of dogs

C

The tendency, during breeding, for birds to produce more offspring than will survive

D

Some female spiders only breeding with males which make the right signals